QIAquick Gel Extraction Kit (250)

For gel extraction/cleanup of up to 10 μg DNA (70 bp to 10 kb) from enzymatic reactions

The QIAquick Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments from gels (up to 400 mg slices) or enzymatic reactions. DNA ranging from 70 bp to 10 kb is purified using a simple and fast bind-wash-elute procedure and an elution volume of 30–50 μl. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column.

Features

• Up to 95% recovery of ready-to-use DNA.
• Fast and convenient procedure.
• Cleanup of DNA up to 10 kb in three easy steps.
• Gel loading dye for convenient sample analysis.

Contents

• QIAquick Spin Columns: 250
• Buffer QG: 2 x 250 ml
• Buffer PE (concentrate): 2 x 55 ml
• Buffer EB: 2 x 15 ml
• Collection Tubes (2 ml): 250
• Loading Dye: 550 µl