HiSpeed Plasmid Midi Kit (25)

For ultrafast purification of up to 750 µg transfection-grade plasmid or cosmid DNA

HiSpeed Plasmid Kits provide fast, large-scale, anion-exchange-based plasmid DNA preparation without centrifugation. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.

Features

• Less than 60 minutes prep time.
• Lysate clearing and isopropanol precipitation without centrifugation.
• No risk of DNA pellet loss during precipitation.
• Up to 750 µg yield of high-copy plasmid DNA.
• LyseBlue for optimum lysis and maximum DNA yield.
• Large-scale plasmid preparation without vacuum manifold.

Contents

• HiSpeed Tips: 25 HiSpeed Midi Tips
• QIAfilter Cartridges: 25 QIAfilter Midi Cartridges
• Caps for QIAfilter: 25
• 20 ml Syringes: 25
• 5 ml Syringes: 25
• QIAprecipitator: 25 QIAprecipitator Midi Modules
• Buffer P1: 250 ml
• Buffer P2: 250 ml
• Buffer P3: 250 ml
• Buffer QBT: 2 x 60 ml
• Buffer QC: 3 x 205 ml
• Buffer QF: 200 ml
• Buffer TE: 30 ml
• LyseBlue®: 250 µl
• RNase A: 25 mg