EndoFree Plasmid Giga Kit (5)

For purification of up to 10 mg endotoxin-free advanced transfection-grade plasmid or cosmid DNA

EndoFree Plasmid Kits provide rapid, anion-exchange-based endotoxin-free plasmid DNA purification. QIAfilter Cartridges enable fast lysate clearing by filtration. The purified DNA exceeds the purity obtained by 2 x CsCl gradient centrifugation and is suitable for advanced transfection-grade applications. The EndoFree Plasmid Buffer Set can be used for preparations of 10 mega or 5 giga transfection-grade plasmid or cosmid DNA preparations.

Features

• Less than 0.1 EU/µg DNA achieved.
• Fast procedure, with integrated endotoxin removal.
• High yields of high-copy plasmid DNA.
• LyseBlue for optimum lysis and maximum DNA yield.

Contents

• QIAGEN-tip 10000: 5
• QIAfilter Mega-Giga Cartridges: 5
• Buffer P1: 3 x 250 ml
• Buffer P2: 3 x 250 ml
• Buffer P3: 3 x 250 ml
• Buffer FWB2: 2 x 140 ml
• Buffer QBT: 2 x 200 ml
• Buffer QC: 7 x 500 ml
• Buffer QN: 510 ml
• Buffer ER: 200 ml
• Buffer TE: 110 ml
• Endotoxin-free water for 70% ethanol: 17 ml
• LyseBlue®: 3 x 250 µl
• RNase A: 3 x 25 mg