QIAGEN Large-Construct Kit (10)

For purification of up to 50 μg BAC, PAC, and P1 DNA or up to 200 μg cosmid DNA, free of genomic DNA

The QIAGEN Large-Construct Kit provides gravity-flow, anion-exchange columns for purification of large-molecular-weight DNA. A unique integrated ATP-dependent exonuclease digestion step ensures selective removal of contaminating genomic DNA. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection.

Features

• Efficient removal of genomic DNA contamination.
• Pure, transfection-grade large-construct DNA.
• Easy and simple procedure.

Contents

• QIAGEN-tip 500: 10
• Buffer P1: 250 ml
• Buffer P2: 250 ml
• Buffer P3: 250 ml
• Buffer QBT: 2 x 60 ml
• Buffer QC: 3 x 240 ml
• Buffer QF: 200 ml
• ATP-Dependent Exonuclease: 10 x 80 μg
• Buffer EX: 110 ml
• Buffer QS: 110 ml
• Exonuclease Solvent: 2 x 1.25 ml
• RNase A (100 mg/ml): 25 mg
• Folded filters: 10
• QIAGEN-tip 500 holders: 10