QIAGEN Plasmid Midi Kit (25)

For purification of up to 10 mg transfection-grade plasmid or cosmid DNA

QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.

The QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. See more information regarding principle below.

Features

• Purity equivalent to 2 x CsCl gradient centrifugation.
• High yields of plasmid DNA.
• Cost-effective preparations.
• LyseBlue for optimum lysis and maximum DNA yield.

Contents

• QIAGEN-tip 100: 25
• Buffer P1: 110 ml 
• Buffer P2: 110 ml
• Buffer P3: 110 ml
• Buffer QBT: 2 x 60 ml
• Buffer QC: 3 x 205 ml
• Buffer QF: 200 ml 
• RNase A: 11 mg
• LyseBlue®: 110 µl