QIAfilter Plasmid Midi Kit (100)

QIAfilter Plasmid Kits

For fast purification of up to 10 mg transfection-grade plasmid or cosmid DNA: QIAfilter Plasmid Kits provide anion-exchange-based plasmid purification with bacterial lysate clearing by filtration. Purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.

Performance

The QIAfilter Plasmid Kits combines QIAfilter Cartridges for rapid clearing of bacterial lysates by filtration instead of centrifugation, with proven QIAGEN-tips containing anion-exchange resin for efficient plasmid purification. Yields of up to 10 mg (giga), 2.5 mg (mega), 500 µg (maxi), and 100 µg (midi) transfection-grade, high-copy plasmid DNA are achieved from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). 

Features

• Lysate clearing without centrifugation
• Reduced plasmid purification time
• High yields of plasmid DNA
• LyseBlue reagent for optimum lysis and maximum DNA yield

Contents

• QIAGEN-tip 100: 100
• QIAfilter Midi Cartridges: 100
• Buffer P1: 4 x 110 ml 
• Buffer P2: 4 x 110 ml
• Buffer P3: 4 x 110 ml
• Buffer QBT: 8 x 60
• Buffer QC: 12 x 205
• Buffer QF: 4 x 200 ml
• RNase A: 4 x11 mg
• LyseBlue®: 4 x110 µl