QIAGEN Genomic-tip 20/G

QIAGEN Genomic-tip 20/G

For isolation of up to 20 µg, 100 µg or 500 µg high-molecular-weight DNA from a wide range of samples: QIAGEN Genomic-tips are gravity-flow, anion-exchange tips that allow efficient purification of genomic DNA from a wide range of biological samples. The purified DNA is sized up to 150 kb with an average size of 50–100 kb.

QIAGEN Genomic-tips come in a range of sizes to suit every preparation need and are supplied with a comprehensive handbook containing detailed protocols for genomic DNA preparation from each sample type. Specific lysis buffers for blood, tissue, cultured cells, yeast and bacteria and all other necessary buffers are provided in the Genomic DNA Buffer Set which can be purchased separately.

Features

• Minimal mechanical shearing allows isolation of high-molecular-weight DNA up to 150 kb in size
• No phenol or chloroform extractions
• Convenient, parallel processing of multiple samples

Performance

The QIAGEN Genomic-tip procedure is very gentle and results in negligible DNA shearing. DNA purified with QIAGEN Genomic-tips is sized up to 150 kb with an average length of 50–100 kb. The DNA is free of all contaminants such as RNA, protein and metabolites and has A260 / A280 ratios between 1.7 and 1.9.

Purified DNA has been used in direct bacterial-genome sequencing.