Invitrogen™ Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 555 Conjugate, 100 µg

Invitrogen™ Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 555 Conjugate, 100 µg

Molecular Probes™ cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us to provide a very high-purity product that is completely free of the toxic A subunit. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside GM1, making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall. When used in neuronal tracing applications, CT-B is typically introduced by pressure injection or by iontophoretic injection into neural tissue.

Features

• Label (Ex/Em): Alexa Fluor™ 555 (555/565 nm)
• At neutral pH, the 11.4 kDa B subunit exists as a 57 kDa pentamer
• Lyophilized product can be dissolved in buffer (e.g., PBS) for use