exoEasy Maxi Kit (20)

exoEasy Maxi Kit

For purification of exosomes and other extracellular vesicles (EVs) from plasma, serum and cell culture supernatant.

The exoEasy Maxi Kit uses membrane affinity spin columns to efficiently isolate exosomes and other extracellular vesicles from serum, plasma, cell culture supernatant and other biological fluids. The maxi column format allows the use of large sample volumes, including up to 4 ml plasma or serum or up to 32 ml cell culture supernatant. The procedure is very fast, consistent and highly suited for isolation of EVs for functional analysis of exosomes and other extracellular vesicles.

Features

• Isolate intact exosomes and other extracellular vesicles
• Suitable for functional testing and physical and biochemical analysis
• Purify exosomes and other extracellular vesicles in 25 minutes
• Process multiple samples in parallel using a simple spin-column procedure
• Use with up to 4 ml of plasma or serum or 32 ml of cell culture supernatant
• Suitable for MISEV-compliant protein characterization

Applications

The exoEasy Kit is highly suited for isolating microvesicles found in serum, plasma, cell culture supernatant and other biological fluids. Isolated vesicles can be analyzed by and used for:

1. Physical characterization (e.g., NTA, TRPS)
2. Electron microscopy
3. Isolation and analysis of DNA, RNA*, proteins, lipids and other constituents. (*For isolation of vesicular RNA, we recommend to use exoRNeasy kits.)
4. Flow cytometry
5. Antibody- or fluorescent dye-based labeling
6. Uptake by recipient cells

Contents

• exoEasy Maxi Spin Columns (in 50 ml Collection Tube): 20 
• Collection Tubes (50 ml): 20 
• Buffer XBP: 3 x 55 ml 
• Buffer XWP: 1 x 200 ml 
• Buffer XE: 45 ml