REPLI-g Mitochondrial DNA Kit (25)

For highly uniform whole genome amplification from human and non-human mitochondrial DNA

The REPLI-g Mitochondrial DNA Kit enables selective amplification of mitochondrial DNA from total DNA samples, without the need for prior mitochondrial DNA isolation. This kit provides DNA polymerase, buffers, and reagents for specific and uniform whole genome amplification from small samples of human and non-human mitochondrial DNA in total DNA samples. For amplification of non-human mtDNA, the REPLI-g Human mt Primer Mix needs to be substituted with an appropriate primer mix suitable for that species (not provided with the kit). The REPLI-g Mitochondrial DNA Kit is based on the multiple displacement amplification technology (MDA), that enriches for mitochondrial DNA with minimal contamination from nuclear DNA, thus avoiding the need for time-consuming isolation of mitochondrial DNA and increasing the sensitivity of downstream assays.

Features

• Sensitive amplification from varying sample types.
• Suitable for amplification of human and non-human mtDNA.
• Overcomes the need for time-consuming isolation of mtDNA.
• Informative results from samples with degraded nuclear DNA.
• DNA amplification from blood cards and hair.

Contents

DNA Polymerase, Buffers, and Reagents for 25 x 50 μl mitochondrial DNA specific whole genome amplification reactions.