QIAGEN Plasmid Midi Kit (100)

For purification of up to 10 mg transfection-grade plasmid or cosmid DNA

QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.

The QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. See more information regarding principle below.

Features

• Purity equivalent to 2 x CsCl gradient centrifugation.
• High yields of plasmid DNA.
• Cost-effective preparations.
• LyseBlue for optimum lysis and maximum DNA yield.

Contents

• QIAGEN-tip 100: 100
• Buffer P1: 500 ml 
• Buffer P2: 500 ml
• Buffer P3: 500 ml
• Buffer QBT: 500 ml
• Buffer QC: 5 x 500 ml
• Buffer QF: 4 x 140 ml 
• RNase A: 50 mg
• LyseBlue®: 500 µl