Applied Biosystems™ ExoSAP-IT™ Express PCR Product Cleanup Reagent, 100 rxns
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Applied Biosystems™ ExoSAP-IT™ Express PCR Product Cleanup Reagent, 100 rxns
ExoSAP-IT™ Express PCR Product Clean-up Reagent, 2000 rxns, Recombinant, Dry Ice, Removes Excess Primers and Unincorporated Nucleotides from a PCR Reaction, Use with PCR Purification, –5 to °30°C Storage Temperature
An enzymatic cleanup method offers precision and speed over technologies such as spin columns or beads. ExoSAP-IT Express reagent consists of two hydrolytic enzymes, a modified Exonuclease I and Shrimp Alkaline Phosphatase (SAP), in a specially formulated buffer. It is used to remove excess primers and dNTPs from a PCR mixture with no interference in downstream applications. This unique, highly stable, one tube solution allows for greater confidence and superior sequencing results.
Features
- 5 minute protocol — Fastest turnaround time to results
- One-tube, one-step PCR cleanup — Add ExoSAP-IT Express directly to PCR product
- Novel enzyme technology — Irreversibly inactivated in just one minute at 80°C
- Conserve PCR samples — 100% recovery of PCR products, regardless of amplicon length
- Eliminate spin columns or beads — Significantly decreases time and errors while increasing yield
- Compatible — No interference in downstream applications
- Scalable — Treat a wide range of volumes
- Multiple formats — Single tube, 8 tube strip, and 96-well plate formats offer flexibility from manual pipetting to automated workflows
- Go green — Generate less waste with a single-tube solution to PCR cleanup
- Research Category: Genetic Diseases
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https://www.thermofisher.com/order/catalog/product/75001.200.UL
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